Journal: PLoS ONE

Article Title: Structural Insight into Inhibitor of Apoptosis Proteins Recognition by a Potent Divalent Smac-Mimetic

doi: 10.1371/journal.pone.0049527

Figure Lengend Snippet: Cytotoxic activity displayed by 9a on MDA-MB-231, HL60 and PC-3 cell lines, determined in three independent experiments (each done in triplicate).

Article Snippet: The MDA-MB-231, HL60 and PC-3 cell lines were obtained from Interlab Cell Line Collection (ICLC, Genova, Italy).

Techniques: Activity Assay

Journal: Journal of Nanobiotechnology

Article Title: Enhanced natural killer cell anti-tumor activity with nanoparticles mediated ferroptosis and potential therapeutic application in prostate cancer

doi: 10.1186/s12951-022-01635-y

Figure Lengend Snippet: Ferumoxytol and NK cells synergistically induce ferroptosis in PC3 prostate cancer and the ferumoxytol mediated ferroptosis activates the cytotoxic function of NK cells. A . Confocal images of C11-BODIPY stained PC3 cells showed lipid peroxidation status. PC3 cells were treated with Ferumoxytol and co-cultured with NK-92MI cells (green). (scale bar = 20 µm) B . Fluorescent level of intracellular LPO in PC3 cells treated with each group was measured by flow cytometry. C . NK cell-mediated tumor cell killing effect of each treatment (ferumoxytol, NK-92MI, and ferumoxytol + NK-92MI) was determined by CFSE/7AAD assay. Ferrostatin-1 ferroptosis inhibitor was used to confirm the ferroptosis enhanced NK cell killing efficacy of the co-treatment ferumoxytol + NK-92MI. D . Interferon gamma secretion in only NK cell treatment and ferumoxytol + NK cell treatment. E . Degranulation of NK-92MI cells was determined by analysis of CD107a expression on NK cells. NK-92MI and PC3 cells were co-cultured at a 10:1 effector: target ratio and measured by flow cytometry. The data represent mean ± s.d. (n = 3) and statistical significance was analyzed by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, and ****P < 0.0001

Article Snippet: NK-92MI (human NK cell line) and PC3 (human prostate cancer cell line) TrampC1 (mouse prostate cancer cell line) were purchased from the American Type Culture Collection (ATCC).

Techniques: Staining, Cell Culture, Flow Cytometry, Expressing, Two Tailed Test

Journal: Journal of Nanobiotechnology

Article Title: Enhanced natural killer cell anti-tumor activity with nanoparticles mediated ferroptosis and potential therapeutic application in prostate cancer

doi: 10.1186/s12951-022-01635-y

Figure Lengend Snippet: A . Expression of ULBPs on prostate cancer cells after Ferumoxytol treatment. Cancer cells were treated with ferumoxytol for 24 h and, expression of ULBPs was measured by flow cytometry. B . MHC class I and II expression on PC-3 prostate cancer cell were determined by flow cytometry C . HMGB1 expression of each treatment was determined by flow cytometry. Cancer cells were co-cultured with mouse primary NK cells with or without ferumoxytol at a 1:1 effector: target ratio, and HMGB1 expression was measured. D . Cell-surface expression of PD-L1 in response to ferumoxytol mediated ferroptosis, as determined by flow cytometry. E . NK cell tumor cell killing effect of each treatment (only NK cells, ferumoxytol + NK cells, and ferumoxytol + NK cells + aPD-L1) was determined by CFSE/7AAD assay. F . Interferon gamma secretion after each treatment (only NK cells, ferumoxytol + NK cells, and ferumoxytol + NK cells + aPD-L1). The data represent mean ± s.d. (n = 3) and statistical significance was analyzed by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: NK-92MI (human NK cell line) and PC3 (human prostate cancer cell line) TrampC1 (mouse prostate cancer cell line) were purchased from the American Type Culture Collection (ATCC).

Techniques: Expressing, Flow Cytometry, Cell Culture, Two Tailed Test

Journal: Journal of Nanobiotechnology

Article Title: Enhanced natural killer cell anti-tumor activity with nanoparticles mediated ferroptosis and potential therapeutic application in prostate cancer

doi: 10.1186/s12951-022-01635-y

Figure Lengend Snippet: Direct anti-cancer activity of combination treatment of NK cells and ferumoxytol in the PC-3 prostate cancer mice model. A . Experimental design of in vivo direct anti-cancer effects. B . Tumor growth curve of each group of treatment. The 6–8-week-old mice were randomly divided into 4 groups, DPBS, NK-92MI, NK-92MI + Fer and NK-92MI + Fer-aPDL1 group. NK cells were intratumorally injected into the PC3 tumors 4 times on days of 14, 18, 21 and 25. C . Representative images of tumors extracted after the experiment. D . Representative H&E images and TUNEL images of each treatment group (scale bar = 50 µm). E . Mean change of body weight of mice during the experiment for each group. The data represent mean ± s.d. (n = 4, 5) and statistical significance was analyzed by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: NK-92MI (human NK cell line) and PC3 (human prostate cancer cell line) TrampC1 (mouse prostate cancer cell line) were purchased from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, In Vivo, Injection, TUNEL Assay, Two Tailed Test